Sterilization is defined as the ability to remove or destroy all forms of microbes, including viruses, bacteria and fungi. Also including bacterial spores, which are highly resistant.

5 Laboratory Sterilization Methods:

  • WET HEAT(Autoclaving)
  • DRY HEAT (Flaming, baking)

Sterilization is usually carried out by autoclave chine, which consists of exposure to steam at 121°C under a pressure of 15 lb/in2 for 15 minutes.

Autoclaves Working Principle:

Autoclaves use pressurized steam as their sterilization agent. The basic concept of an autoclave is to have each item sterilized -whether it is a liquid, plastic ware, or glassware- come in direct contact with steam at a specific temperature and pressure for a specific amount of time.

Autoclaves provide a physical method for disinfection and sterilization. Autoclaves operate at high temperature and pressure in order to kill microorganisms and spores. They are used to sterilize certain biological waste, media, instruments and lab ware.

Importance of sterilization

Sound sterilization practices in dentistry protect patients, dentists and the whole dental team. They prevent the growth of bacteria on instruments

Improper sterilization leads to?

Microorganisms in blood and saliva can carry bacteria and diseases, When proper sterilization procedures aren’t used they can pass from one patient to another.